| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Specificity: |
This antibody reacts specifically with RNA polymerase II CTD repeat YSPTSPS (Ser5) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser5 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5 |
| Gene ID: |
6193 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition, Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, At the start of transcription, During transcription elongation, Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), Forms the polymerase active center together with the second largest subunit, It is composed of mobile elements that move relative to each other, Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs, Pol II is the central component of the basal RNA polymerase II transcription machinery, Pol II moves on the template as the transcript elongates, RPB1 is part of the core element with the central large cleft, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II, acting both as a replicate and transcriptase for the viral RNA circular genome, elongation, termination and mRNA processing, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template, which serves as a platform for assembly of factors that regulate transcription initiation, DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Other name: |
Anti-RNA polymerase II CTD repeat YSPTSPS (Ser5) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
RNA polymerase II CTD repeat YSPTSPS (Ser5) |
| Short name: |
Anti-RNA polymerase II CTD repeat YSPTSPS (Ser5) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RNA polymerizing enzyme II CTD repeat YSPTSPS (Ser5) |
| Identity: |
2497 |
| Gene: |
CTD |
More about : CTD |
| Long gene name: |
Coats disease |
| Locus: |
reserved |
| Discovery year: |
1994-01-05 |
| Entrez gene record: |
1283 |
| Pubmed identfication: |
11884883 10484772 |