| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RAB5 |
| Specificity: |
This antibody reacts specifically with RAB5 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RAB5 |
| Gene ID: |
5868 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CXC, Due to the presence of truncated sequences in this CD, GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP, Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state, In mammals, In yeast, Lipid binding is essential for membrane attachment, Most Rab GTPases contain a lipid modification site at the C-terminus, Rab5 GTPases localize to early endosomes and regulate fusion of clathrin-coated vesicles to early endosomes and fusion between early endosomes, Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), The members of this subfamily are involved in endocytosis and endocytic-sorting pathways, This subfamily includes Rab5 and Rab22 of mammals, Ypt51/Ypt52/Ypt53 of yeast, Ypt51p family members similarly regulate membrane trafficking through prevacuolar compartments, a key feature of most Rab proteins, and RabF of plants, or CCX, the lipid modification site is not available for annotation, which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization, with sequence motifs CC, Rab5-related subfamily |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
RAB5 Polyclonal Antibody |
| Other name: |
Anti-RAB5 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RAB5 |
| Short name: |
Anti-RAB5 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RAB5 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9783 |
| Gene: |
RAB5A |
More about : RAB5A |
| Long gene name: |
RAB5A, member RAS oncogene family |
| Synonyms gene: |
RAB5 |
| Synonyms name: |
RAS-associated protein RAB5A |
| Locus: |
3p24, 3 |
| Discovery year: |
1990-01-22 |
| Entrez gene record: |
5868 |
| Pubmed identfication: |
1999336 |
| RefSeq identity: |
NM_004162 |
| Classification: |
RAB, member RAS oncogene GTPases |
| Havana BLAST/BLAT: |
OTTHUMG00000129889 |