| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Rab5 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Rab5 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Rab5 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P20339 |
| Purity: |
Antigen affinity |
| Description: |
Activation of the protein was either continuous or repetitive for up to 10 minutes, Ectopically expressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal compartments and stimulated the enlargement of endosomes in cultured cortical neurons, Expression of a dominant-negative mutant delayed this collapse of apoptotic thymocytes, It signals to the actin cytoskeleton through RNTRE, RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes, RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway, The protein is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling, Tuberin functions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis, a RAB5-specific GTPase-activating protein(GAP), also called RAB5, but it ended before the collapse of engulfed apoptotic cells, is a protein that in humans is encoded by the RAB5A gene, showing a role for RAB5 in phagosome maturation, Ras-associated protein 5A |
| Immunogen: |
mouse and rat), Amino acids 138-155 (ANKRAVDFQEAQSYADDN) were used as the immunogen for this Rab5 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Rab5 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Rab5 |
| Short name: |
Rab5 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Rab5 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9783 |
| Gene: |
RAB5A |
More about : RAB5A |
| Long gene name: |
RAB5A, member RAS oncogene family |
| Synonyms gene: |
RAB5 |
| Synonyms name: |
RAS-associated protein RAB5A |
| Locus: |
3p24, 3 |
| Discovery year: |
1990-01-22 |
| Entrez gene record: |
5868 |
| Pubmed identfication: |
1999336 |
| RefSeq identity: |
NM_004162 |
| Classification: |
RAB, member RAS oncogene GTPases |
| Havana BLAST/BLAT: |
OTTHUMG00000129889 |