| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NG2 |
| Specificity: |
This antibody reacts specifically with NG2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NG2 |
| Gene ID: |
1464 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ACK1 and BCAR1, In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, May activate FAK and ERK1/ERK2 signaling cascades, NG2 also stimulates alpha-4, NG2 is a proteoglycan found in the nervous system, They vary in their cellular locations and have diverse structure and functions, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, but is also associated with developing chondrocytes, cardiomyocytes, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain, pericytes and several human tumours, Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NG2 G16 RING5 RMA1 |
| Also known as: |
NG2 Polyclonal Antibody |
| Other name: |
Anti-NG2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NG2 |
| Short name: |
Anti-NG2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NG2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10068 |
| Gene: |
RNF5 |
More about : RNF5 |
| Long gene name: |
ring finger protein 5 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , ring finger protein 5 |
| Locus: |
6p21, 32 |
| Discovery year: |
1997-10-16 |
| GenBank acession: |
U89336 |
| Entrez gene record: |
6048 |
| Pubmed identfication: |
9533025 |
| RefSeq identity: |
NM_006913 |
| Classification: |
Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000031093 |