| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CDK2 Thr14 |
| Specificity: |
This antibody reacts specifically with CDK2 (Thr14) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr14 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr14 [EG(p-T)YG] |
| Gene ID: |
1017 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B), It is a catalytic subunit of the cyclin-dependent protein kinase complex, Its activity is also regulated by its protein phosphorylation, This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, This protein kinase is highly similar to the gene products of S, Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported, and S, and essential for cell cycle G1/S phase transition, cdk2 binds cyclin type A and E proteins and controls progression into S-phase, cdk2 is a cell cycle protein closely related to Cdc2 (cdk1) that has proved useful as a marker of proliferation, cerevisiae cdc28, pombe cdc2, whose activity is restricted to the G1-S phase, The protein encoded by this gene is a member of the Ser/Thr protein kinase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
CDK2 (Thr14) Polyclonal Antibody |
| Other name: |
Anti-CDK2 (Thr14) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CDK2 (Thr14) |
| Short name: |
Anti-CDK2 (Thr14) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CDK2 (Thr14) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1771 |
| Gene: |
CDK2 |
More about : CDK2 |
| Long gene name: |
cyclin dependent kinase 2 |
| Locus: |
12q13, 2 |
| Discovery year: |
1992-02-28 |
| GenBank acession: |
M68520 |
| Entrez gene record: |
1017 |
| Pubmed identfication: |
1717994 8275715 |
| Classification: |
Cyclin dependent kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000170575 |