| Long name: |
Biotin Conjugated, cdc2 (Thr14) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
cdc2 Thr14 |
| Specificity: |
This is a highly specific antibody against cdc2 Thr14 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr14 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human cdc2 around the phosphorylation site of Thr14 [EG(p-T)YG] |
| Gene ID number: |
983 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161, Cdc2 exists as a complex with both cyclin A and cyclin B, Furthermore, However, Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase, The best characterized of these associations is the Cdc2 p34 cyclin B complex, The isoform CDC2deltaT is found in breast cancer tissues, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration, plays a key role in the control of the eukaryotic cell cycle, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14, where it is required for entry into S phase and mitosis, which is required for the G2 to M phase transition, The cell division control protein cdc2 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
CDK 1, CDK1, Cdc 2, Cell Divsion Cycle 2 Protein, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, P34CDC2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
cdc2 (Thr14) Conjugated |
| Short name: |
Conjugated, cdc2 (Thr14) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, cdc2 (Thr14) (Antibody to) |
| Alternative technique: |
antibodies |