| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CARM1 |
| Specificity: |
This antibody reacts specifically with CARM1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CARM1 |
| Gene ID: |
10498 |
| Swiss Prot: |
Q86X55 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as coactivator for PPARG, Also methylates arginine residues in RNA-binding proteins PABPC1, Also seems to be involved in p53/TP53 transcriptional activation, During monocyte inflammatory stimulation, During myogenic transcriptional activation, During nuclear hormone receptor activation and TCF7L2/TCF4 activation, ELAVL1 and ELAV4, Methylates EP300/P300, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription, Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors, Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, acts together with EP300/P300 as a coactivator for NF-kappa-B, acts together with NCOA3/ACTR as a coactivator for MEF2C, and at 'Arg-580' and 'Arg-604' in the KIX domain, and mRNA stability, both at 'Arg-2142', forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling, methylates histone H3 at 'Arg-17' (H3R17me), pre-mRNA splicing, promotes adipocyte differentiation and the accumulation of brown fat tissue, transcription regulation, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs, which may loosen its interaction with NCOA2/GRIP1, Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PRMT4 |
| Also known as: |
CARM1 Polyclonal Antibody |
| Other name: |
Anti-CARM1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CARM1 |
| Short name: |
Anti-CARM1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CARM1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
23393 |
| Gene: |
CARM1 |
More about : CARM1 |
| Long gene name: |
coactivator associated arginine methyltransferase 1 |
| Locus: |
19p13, 2 |
| Discovery year: |
2003-11-14 |
| GenBank acession: |
AF055027 |
| Entrez gene record: |
10498 |
| Pubmed identfication: |
10381882 11724789 |
| RefSeq identity: |
NM_199141 |
| Classification: |
Protein arginine methyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000180699 |