| Long name: |
FITC Conjugated, CARM1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CARM1 |
| Specificity: |
This is a highly specific antibody against CARM1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
430-480/608 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human CARM1 |
| Gene ID number: |
10498 |
| Swiss Prot: |
Q86X55 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts as coactivator for PPARG, Also methylates arginine residues in RNA-binding proteins PABPC1, Also seems to be involved in p53/TP53 transcriptional activation, During monocyte inflammatory stimulation, During myogenic transcriptional activation, During nuclear hormone receptor activation and TCF7L2/TCF4 activation, ELAVL1 and ELAV4, Methylates EP300/P300, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription, Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors, Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, acts together with EP300/P300 as a coactivator for NF-kappa-B, acts together with NCOA3/ACTR as a coactivator for MEF2C, and at 'Arg-580' and 'Arg-604' in the KIX domain, and mRNA stability, both at 'Arg-2142', forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling, methylates histone H3 at 'Arg-17' (H3R17me), pre-mRNA splicing, promotes adipocyte differentiation and the accumulation of brown fat tissue, transcription regulation, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs, which may loosen its interaction with NCOA2/GRIP1, Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
PRMT4 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CARM1 Conjugated |
| Short name: |
FITC Conjugated, CARM1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, CARM1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
23393 |
| Gene: |
CARM1 |
More about : CARM1 |
| Long gene name: |
coactivator associated arginine methyltransferase 1 |
| Locus: |
19p13, 2 |
| Discovery year: |
2003-11-14 |
| GenBank acession: |
AF055027 |
| Entrez gene record: |
10498 |
| Pubmed identfication: |
10381882 11724789 |
| RefSeq identity: |
NM_199141 |
| Classification: |
Protein arginine methyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000180699 |