| Long name: |
FITC Conjugated, AMPK gamma 3/PRKAG3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AMPK gamma 3/PRKAG3 |
| Specificity: |
This is a highly specific antibody against AMPK gamma 3/PRKAG3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
174-220/489 |
| Source: |
KLH conjugated synthetic peptide derived from human AMPK gamma 3 |
| Gene ID number: |
53632 |
| Swiss Prot: |
Q9UGI9 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ADP also stimulates phosphorylation, ADP and ATP, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, AMPK acts via direct phosphorylation of metabolic enzymes, ATP promotes dephosphorylation of catalytic subunit, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, Gamma non-catalytic subunit mediates binding to AMP, In response to reduction of intracellular ATP levels, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism, and by longer-term effects via phosphorylation of transcription regulators, as well as cell growth and proliferation, carbohydrate and lipid biosynthesis, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits, probably by indirectly activating myosin, rendering the AMPK enzyme inactive, without stimulating already phosphorylated catalytic subunit, AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AMPK gamma 3/PRKAG3 Conjugated |
| Short name: |
FITC Conjugated, AMPK gamma 3/PRKAG3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
AMP-activated, fluorecein coupled, g 3 non-catalytic subunit (Antibody to), AMPK g 3/protein phosphorylation catalyst |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AMP-activated, Extracellular, PRKAG3 and IDBG-643891 and ENSBTAG00000013492 and 511961, PRKAG3 and IDBG-81160 and ENSG00000115592 and 53632, Prkag3 and IDBG-168633 and ENSMUSG00000006542 and 241113, adenyl nucleotide binding, gamma 3 non-catalytic subunit, this GO :0004679 and AMP-activated protein kinase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0005978 and glycogen biosynthetic process and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006633 and fatty acid biosynthetic process and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0015758 and glucose transport and biological process this GO :0016301 and kinase activity and molecular function this GO :0016310 and phosphorylation and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030554 and adenyl nucleotide binding and molecular function this GO :0031588 and AMP-activated protein kinase complex and cellular component this GO :0035556 and intracellular signal transduction and biological process this GO :0061024 and membrane organization and biological process, this GO :0004679 : AMP-activated protein kinase activity, this GO :0004679 : AMP-activated protein kinase activity and also this GO :0005524 : ATP binding and also this GO :0016301 : kinase activity and also this GO :0019901 : protein kinase binding and also this GO :0030554 : adenyl nucleotide binding, this GO :0005524 : ATP binding, this GO :0016301 : kinase activity, this GO :0019901 : protein kinase binding, this GO :0030554 : adenyl nucleotide binding, protein kinase |
| Identity: |
9387 |
| Gene: |
PRKAG3 |
More about : PRKAG3 |
| Long gene name: |
protein kinase AMP-activated non-catalytic subunit gamma 3 |
| Synonyms gene name: |
AMP-activated, gamma 3 non-catalytic subunit , protein kinase |
| Locus: |
2q35 |
| Discovery year: |
2000-07-31 |
| GenBank acession: |
AF214519 |
| Entrez gene record: |
53632 |
| Pubmed identfication: |
10818001 |
| Havana BLAST/BLAT: |
OTTHUMG00000133078 |