| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CHK2 Thr68 |
| Specificity: |
This antibody reacts specifically with CHK2 (Thr68) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr68 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68 |
| Gene ID: |
11200 |
| Swiss Prot: |
O96017 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1, Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1, CDC25B and CDC25C, Following activation, Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, Its absence may be a cause of the chromosomal instability observed in some cancer cells, MDM4 and PML, May also negatively regulate cell cycle progression during unperturbed cell cycles, May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest, Phosphorylation of MDM4 may also reduce degradation of p53/TP53, Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, Regulates DNA repair through phosphorylation of BRCA2, Regulates apoptosis through the phosphorylation of p53/TP53, Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, Tumor suppressor, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination, inhibiting their activity, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1, leading to accumulation of active p53/TP53, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T], Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CDS1 CHK2 HuCds1 PP1425 bA444G7 |
| Also known as: |
CHK2 (Thr68) Polyclonal Antibody |
| Other name: |
Anti-CHK2 (Thr68) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CHK2 (Thr68) |
| Short name: |
Anti-CHK2 (Thr68) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CHK2 (Thr68) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
16627 |
| Gene: |
CHEK2 |
More about : CHEK2 |
| Long gene name: |
checkpoint kinase 2 |
| Synonyms gene: |
RAD53 |
| Synonyms gene name: |
CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S |
| Locus: |
22q12, 1 |
| Discovery year: |
2001-09-19 |
| GenBank acession: |
AF086904 |
| Entrez gene record: |
11200 |
| Pubmed identfication: |
9836640 10097108 |
| RefSeq identity: |
NM_001005735 |
| Havana BLAST/BLAT: |
OTTHUMG00000151023 |
| Locus Specific Databases: |
LRG_302 |