| Long name: |
Cy7 Conjugated, CHK2 (Thr68) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Cy7 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CHK2 Thr68 |
| Specificity: |
This is a highly specific antibody against CHK2 Thr68 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr68 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
50-90/543 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68 |
| Gene ID number: |
11200 |
| Swiss Prot: |
O96017 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1, Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1, CDC25B and CDC25C, Following activation, Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, Its absence may be a cause of the chromosomal instability observed in some cancer cells, MDM4 and PML, May also negatively regulate cell cycle progression during unperturbed cell cycles, May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest, Phosphorylation of MDM4 may also reduce degradation of p53/TP53, Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, Regulates DNA repair through phosphorylation of BRCA2, Regulates apoptosis through the phosphorylation of p53/TP53, Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, Tumor suppressor, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination, inhibiting their activity, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1, leading to accumulation of active p53/TP53, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T], Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
743nm/767nm |
| Synonyms: |
CDS1 CHK2 HuCds1 PP1425 bA444G7 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Conjugated: |
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths |
| Gene target: |
CHK2 (Thr68) Conjugated |
| Short name: |
Conjugated, CHK2 (Thr68) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy7 |
| Alternative name: |
cyanine 7 coupled, CHK2 (Thr68) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16627 |
| Gene: |
CHEK2 |
More about : CHEK2 |
| Long gene name: |
checkpoint kinase 2 |
| Synonyms gene: |
RAD53 |
| Synonyms gene name: |
CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S |
| Locus: |
22q12, 1 |
| Discovery year: |
2001-09-19 |
| GenBank acession: |
AF086904 |
| Entrez gene record: |
11200 |
| Pubmed identfication: |
9836640 10097108 |
| RefSeq identity: |
NM_001005735 |
| Havana BLAST/BLAT: |
OTTHUMG00000151023 |
| Locus Specific Databases: |
LRG_302 |