| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Aurora A Thr288 |
| Specificity: |
This antibody may detect this protein in mouse when phosphorylated at Thr279, but at a reduced affinity due to a single amino acid change in the immunogen, Aurora A (Thr288), This phosphorylation site is homologous to that of Thr281 in Rat |
| Modification: |
Phosphorylation |
| Modification site: |
Thr288 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Aurora A around the phosphorylation site of Thr288 |
| Gene ID: |
6790 |
| Swiss Prot: |
O14965 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also acts as a key regulatory component of the p53/TP53 pathway, Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, BORA, BRCA1, CDC25B, DLGP5, HDAC6, Important for microtubule formation and/or stabilization, KIF2A, LATS2, NDEL1, Necessary for proper cilia disassembly prior to mitosis, PARD3, PLK1, PPP1R2, Phosphorylates its own inhibitors, Phosphorylates numerous target proteins, Plays a role in microtubule remodeling during neurite extension, RASSF1, Regulates KIF2A tubulin depolymerase activity, Required for initial activation of CDK1 at centrosomes, Required for normal axon formation, TACC3, and cytokinesis, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, including ARHGEF2, p53/TP53 and TPX2, spindle assembly checkpoint, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity, Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AIRK1, ARK-1, ARK1, AURA, AURKA, AURORA2, AYK1, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, IAK1, PPP1R47, STK15, STK6, STK7, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, hARK1, AIK |
| Also known as: |
Aurora A (Thr288) Polyclonal Antibody |
| Other name: |
Anti-Aurora A (Thr288) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Aurora A (Thr288) |
| Short name: |
Anti-Aurora A (Thr288) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Aurora A (Thr288) (polyclonal) |
| Alternative technique: |
polyclonals |