| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MSK1/2 |
| Specificity: |
This antibody reacts specifically with MSK1/2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MSK1 |
| Gene ID: |
8986, 9252 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATF1 H3 and HMG14 in response to mitogen and stress, Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), In lipopolysaccharide-stimulated primary macrophages, It is also known to be required for the phosphorylation of CREB, MSK1 autophosphorylate on at least 5 sites, MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase, MSK1 contains two kinase domains (N term and a C term), MSK2 plays an essential role in the control of RELA transcriptional activity in response to TNF, May also phosphorylate 'Ser-28' of histone H3, Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14), Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain, Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, Similar to RSK, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines, including proto-oncogenes c-fos/FOS and c-jun/JUN, stress stimuli and EGF, via CREB1 and ATF1 transcription factors, which results in the transcriptional activation of several immediate early genes, MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MSK1 RLPK |
| Also known as: |
MSK1/2 Polyclonal Antibody |
| Other name: |
Anti-MSK1/2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MSK1/2 |
| Short name: |
Anti-MSK1/2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MSK1/2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10434 |
| Gene: |
RPS6KA5 |
More about : RPS6KA5 |
| Long gene name: |
ribosomal protein S6 kinase A5 |
| Synonyms gene name: |
90kD, polypeptide 5 , ribosomal protein S6 kinase |
| Locus: |
14q32, 11 |
| Discovery year: |
1999-03-10 |
| GenBank acession: |
AF074393 |
| Entrez gene record: |
9252 |
| Pubmed identfication: |
9687510 10702687 |
| RefSeq identity: |
NM_004755 |
| Classification: |
Mitogen-activated protein kinase-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000171055 |