| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MSK1 Ser376 |
| Specificity: |
This antibody reacts specifically with MSK1 (Ser376) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser376 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Ser376 [GY(p-S)FV] |
| Gene ID: |
9252 |
| Swiss Prot: |
O75582 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATF1 H3 and HMG14 in response to mitogen and stress, It is also known to be required for the phosphorylation of CREB, MSK1 autophosphorylate on at least 5 sites, MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase, MSK1 contains two kinase domains (N term and a C term), Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain, Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, Similar to RSK, MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MSK1 RLPK |
| Also known as: |
MSK1 (Ser376) Polyclonal Antibody |
| Other name: |
Anti-MSK1 (Ser376) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MSK1 (Ser376) |
| Short name: |
Anti-MSK1 (Ser376) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MSK1 (Ser376) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10434 |
| Gene: |
RPS6KA5 |
More about : RPS6KA5 |
| Long gene name: |
ribosomal protein S6 kinase A5 |
| Synonyms gene name: |
90kD, polypeptide 5 , ribosomal protein S6 kinase |
| Locus: |
14q32, 11 |
| Discovery year: |
1999-03-10 |
| GenBank acession: |
AF074393 |
| Entrez gene record: |
9252 |
| Pubmed identfication: |
9687510 10702687 |
| RefSeq identity: |
NM_004755 |
| Classification: |
Mitogen-activated protein kinase-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000171055 |