| Long name: |
ALEXA FLUOR 647 Conjugated, MSK1/2 Polyclonal Antibody |
| Also known as: |
Anti-MSK1/2 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MSK1/2 |
| Specificity: |
This is a highly specific antibody against MSK1/2 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
49-94/802 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MSK1 |
| Gene ID Number: |
8986, 9252 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
ATF1 H3 and HMG14 in response to mitogen and stress, Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), In lipopolysaccharide-stimulated primary macrophages, It is also known to be required for the phosphorylation of CREB, MSK1 autophosphorylate on at least 5 sites, MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase, MSK1 contains two kinase domains (N term and a C term), MSK2 plays an essential role in the control of RELA transcriptional activity in response to TNF, May also phosphorylate 'Ser-28' of histone H3, Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14), Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain, Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, Similar to RSK, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines, including proto-oncogenes c-fos/FOS and c-jun/JUN, stress stimuli and EGF, via CREB1 and ATF1 transcription factors, which results in the transcriptional activation of several immediate early genes, MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
MSK1 RLPK |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the MSK1/2 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
MSK1/2 |
| Short name: |
MSK1/2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, MSK1/2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10434 |
| Gene: |
RPS6KA5 |
More about : RPS6KA5 |
| Long gene name: |
ribosomal protein S6 kinase A5 |
| Synonyms gene name: |
90kD, polypeptide 5 , ribosomal protein S6 kinase |
| Locus: |
14q32, 11 |
| Discovery year: |
1999-03-10 |
| GenBank acession: |
AF074393 |
| Entrez gene record: |
9252 |
| Pubmed identfication: |
9687510 10702687 |
| RefSeq identity: |
NM_004755 |
| Classification: |
Mitogen-activated protein kinase-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000171055 |