| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BRG-1/SMARCA4/SNF2 beta |
| Specificity: |
This antibody reacts specifically with Transcription activator BRG1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human of human SMARCA4/Transcription activator BRG1 |
| Gene ID: |
6597 |
| Swiss Prot: |
P51532 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As neural progenitors exit mitosis and differentiate into neurons, At the same time, Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex), Component of the CREST-BRG1 complex, During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state, In resting neurons, RB1 is dephosphorylated by calcineurin, The CREST-BRG1 complex also binds to the NR2B promoter, The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth, The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells, The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes, Upon calcium influx, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF), npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex, which leads to release of the repressor complex, which leads to transcriptional activation, Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
hSNF2b BRG1 BAF190 SNF2 SWI2 SNF2-BETA SNF2LB FLJ39786 |
| Also known as: |
Transcription activator BRG1 Antibody |
| Other name: |
Anti-Transcription activator BRG1 |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
Transcription activator BRG1 |
| Short name: |
Fluor 594, Anti-Transcription activator BRG1 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Transcription activator BRG1 |
| Identity: |
11100 |
| Gene: |
SMARCA4 |
More about : SMARCA4 |
| Long gene name: |
SWI/SNF related, actin dependent regulator of chromatin, matrix associated, member 4 , subfamily a |
| Synonyms gene: |
SNF2L4 |
| Synonyms name: |
SNF2-like 4 global transcription activator homologous sequence sucrose nonfermenting-like 4 mitotic growth and transcription activator BRM/SWI2-related gene 1 homeotic gene regulator nuclear protein GRB1 brahma protein-like 1 ATP-dependent helicase SMARCA4 |
| Locus: |
19p13, 2 |
| Discovery year: |
1995-07-17 |
| GenBank acession: |
D26156 |
| Entrez gene record: |
6597 |
| Pubmed identfication: |
8208605 |
| RefSeq identity: |
NM_003072 |
| Havana BLAST/BLAT: |
OTTHUMG00000169272 |
| Locus Specific Databases: |
LRG_878 |