| Long name: |
ALEXA FLUOR 647 Conjugated, Transcription activator BRG1 Antibody |
| Also known as: |
Anti-BRG-1/SMARCA4/SNF2 beta PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BRG-1/SMARCA4/SNF2 beta |
| Specificity: |
This is a highly specific antibody against BRG-1/SMARCA4/SNF2 beta |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
65-115/1647 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human of human SMARCA4/Transcription activator BRG1 |
| Gene ID Number: |
6597 |
| Swiss Prot: |
P51532 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
As neural progenitors exit mitosis and differentiate into neurons, At the same time, Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex), Component of the CREST-BRG1 complex, During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state, In resting neurons, RB1 is dephosphorylated by calcineurin, The CREST-BRG1 complex also binds to the NR2B promoter, The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth, The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells, The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes, Upon calcium influx, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF), npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex, which leads to release of the repressor complex, which leads to transcriptional activation, Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
hSNF2b BRG1 BAF190 SNF2 SWI2 SNF2-BETA SNF2LB FLJ39786 |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the Transcription activator BRG1 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
Transcription activator BRG1 |
| Short name: |
Transcription activator BRG1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, Transcription activator BRG1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11100 |
| Gene: |
SMARCA4 |
More about : SMARCA4 |
| Long gene name: |
SWI/SNF related, actin dependent regulator of chromatin, matrix associated, member 4 , subfamily a |
| Synonyms gene: |
SNF2L4 |
| Synonyms name: |
SNF2-like 4 global transcription activator homologous sequence sucrose nonfermenting-like 4 mitotic growth and transcription activator BRM/SWI2-related gene 1 homeotic gene regulator nuclear protein GRB1 brahma protein-like 1 ATP-dependent helicase SMARCA4 |
| Locus: |
19p13, 2 |
| Discovery year: |
1995-07-17 |
| GenBank acession: |
D26156 |
| Entrez gene record: |
6597 |
| Pubmed identfication: |
8208605 |
| RefSeq identity: |
NM_003072 |
| Havana BLAST/BLAT: |
OTTHUMG00000169272 |
| Locus Specific Databases: |
LRG_878 |