| Long name: |
Transcription activator BRG1 Antibody |
| Also known as: |
Transcription activator BRG1 Antibody |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
BRG-1/SMARCA4/SNF2 beta |
| Specificity: |
This is a highly specific antibody against BRG-1/SMARCA4/SNF2 beta |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
65-115/1647 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human of human SMARCA4/Transcription activator BRG1 |
| Gene ID number: |
6597 |
| Swiss Prot: |
P51532 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
As neural progenitors exit mitosis and differentiate into neurons, At the same time, Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex), Component of the CREST-BRG1 complex, During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state, In resting neurons, RB1 is dephosphorylated by calcineurin, The CREST-BRG1 complex also binds to the NR2B promoter, The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth, The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells, The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes, Upon calcium influx, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF), npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex, which leads to release of the repressor complex, which leads to transcriptional activation, Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
ATP-dependent helicase SMARCA4, BAF190, BAF190A, BRG1-associated factor 190A, MRD16, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, RTPS2, SMARCA4, SNF2, SNF2-beta, SNF2B, SNF2L4, SNF2LB, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, SWI2, Transcription activator BRG1, hSNF2b, BRG1 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Transcription activator BRG1 |
| Short name: |
Transcription activator BRG1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Transcription activator BRG1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11100 |
| Gene: |
SMARCA4 |
More about : SMARCA4 |
| Long gene name: |
SWI/SNF related, actin dependent regulator of chromatin, matrix associated, member 4 , subfamily a |
| Synonyms gene: |
SNF2L4 |
| Synonyms: |
hSNF2b BRG1 BAF190 SNF2 SWI2 SNF2-BETA SNF2LB FLJ39786 |
| Synonyms name: |
SNF2-like 4 global transcription activator homologous sequence sucrose nonfermenting-like 4 mitotic growth and transcription activator BRM/SWI2-related gene 1 homeotic gene regulator nuclear protein GRB1 brahma protein-like 1 ATP-dependent helicase SMARCA4 |
| Locus: |
19p13, 2 |
| Discovery year: |
1995-07-17 |
| GenBank acession: |
D26156 |
| Entrez gene record: |
6597 |
| Pubmed identfication: |
8208605 |
| RefSeq identity: |
NM_003072 |
| Havana BLAST/BLAT: |
OTTHUMG00000169272 |
| Locus Specific Databases: |
LRG_878 |