| Long name: |
FITC Conjugated, Transcription activator BRG1 Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BRG-1/SMARCA4/SNF2 beta |
| Specificity: |
This is a highly specific antibody against BRG-1/SMARCA4/SNF2 beta |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
65-115/1647 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human of human SMARCA4/Transcription activator BRG1 |
| Gene ID number: |
6597 |
| Swiss Prot: |
P51532 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
As neural progenitors exit mitosis and differentiate into neurons, At the same time, Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex), Component of the CREST-BRG1 complex, During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state, In resting neurons, RB1 is dephosphorylated by calcineurin, The CREST-BRG1 complex also binds to the NR2B promoter, The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth, The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells, The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes, Upon calcium influx, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF), npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex, which leads to release of the repressor complex, which leads to transcriptional activation, Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
hSNF2b BRG1 BAF190 SNF2 SWI2 SNF2-BETA SNF2LB FLJ39786 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Transcription activator BRG1 Conjugated |
| Short name: |
FITC Conjugated, Transcription activator BRG1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Transcription activator BRG1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11100 |
| Gene: |
SMARCA4 |
More about : SMARCA4 |
| Long gene name: |
SWI/SNF related, actin dependent regulator of chromatin, matrix associated, member 4 , subfamily a |
| Synonyms gene: |
SNF2L4 |
| Synonyms name: |
SNF2-like 4 global transcription activator homologous sequence sucrose nonfermenting-like 4 mitotic growth and transcription activator BRM/SWI2-related gene 1 homeotic gene regulator nuclear protein GRB1 brahma protein-like 1 ATP-dependent helicase SMARCA4 |
| Locus: |
19p13, 2 |
| Discovery year: |
1995-07-17 |
| GenBank acession: |
D26156 |
| Entrez gene record: |
6597 |
| Pubmed identfication: |
8208605 |
| RefSeq identity: |
NM_003072 |
| Havana BLAST/BLAT: |
OTTHUMG00000169272 |
| Locus Specific Databases: |
LRG_878 |