| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SKAP55 |
| Specificity: |
This antibody reacts specifically with SKAP55 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SKAP55 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells, SKAP55 associates with SLAP-130, SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines, SKAP55-R, The human SKAP55 gene maps to chromosome 17q21, another component of the Fyn complex, contains a coiled-coil N-terminal domain, similar in overall structure to SKAP55, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation, 32 and encodes a 359 amino acid protein, Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SKAP55 |
| Also known as: |
SKAP55 Polyclonal Antibody |
| Other name: |
Anti-SKAP55 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SKAP55 |
| Short name: |
Anti-SKAP55 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SKAP55 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
15605 |
| Gene: |
SKAP1 |
More about : SKAP1 |
| Long gene name: |
src kinase associated phosphoprotein 1 |
| Synonyms gene: |
SCAP1 |
| Synonyms gene name: |
src family associated phosphoprotein 1 |
| Locus: |
17q21, 32 |
| Discovery year: |
2001-06-21 |
| GenBank acession: |
Y11215 |
| Entrez gene record: |
8631 |
| Pubmed identfication: |
9195899 |
| RefSeq identity: |
NM_003726 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000178823 |