| Long name: |
FITC Conjugated, SKAP55 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SKAP55 |
| Specificity: |
This is a highly specific antibody against SKAP55 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human SKAP55 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells, SKAP55 associates with SLAP-130, SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines, SKAP55-R, The human SKAP55 gene maps to chromosome 17q21, another component of the Fyn complex, contains a coiled-coil N-terminal domain, similar in overall structure to SKAP55, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation, 32 and encodes a 359 amino acid protein, Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SKAP55 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
SKAP55 Conjugated |
| Short name: |
FITC Conjugated, SKAP55 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, SKAP55 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
15605 |
| Gene: |
SKAP1 |
More about : SKAP1 |
| Long gene name: |
src kinase associated phosphoprotein 1 |
| Synonyms gene: |
SCAP1 |
| Synonyms gene name: |
src family associated phosphoprotein 1 |
| Locus: |
17q21, 32 |
| Discovery year: |
2001-06-21 |
| GenBank acession: |
Y11215 |
| Entrez gene record: |
8631 |
| Pubmed identfication: |
9195899 |
| RefSeq identity: |
NM_003726 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000178823 |