| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DNA Polymerase iota |
| Specificity: |
This antibody reacts specifically with DNA Polymerase iota |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DNA Polymerase iota |
| Gene ID: |
11201 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
At thymine templates, DNA Pol i acts sequentially with DNA Pol Î, DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, DNA polymerases can often bypass DNA lesions that block DNA replication, One such DNA polymerase is the distributive enzyme DNA Pol i, POLI is located on human chromosome 18q21, Therefore, a region often implicated in the etiology of many human cancers, adenosine, recombination and mutagenesis, repair, replication involving DNA Pol i is likely to be highly mutagenic, thereby allowing the replication of damaged DNA, to complete the DNA lesion bypass, which is encoded by the POLI gene, which is essential for damage-induced mutagenesis, ©, 2, DNA polymerase activity is essential for replication |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
POLI |
| Also known as: |
DNA Polymerase iota Polyclonal Antibody |
| Other name: |
Anti-DNA Polymerase iota Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DNA Polymerase iota |
| Short name: |
Anti-DNA Polymerase iota (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Desoxyribonucleic acid polymerizing enzyme iota (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9815 |
| Gene: |
RAD30B |
More about : RAD30B |
| Long gene name: |
DNA polymerase iota |
| Synonyms gene: |
RAD3OB |
| Synonyms gene name: |
polymerase (DNA directed) iota polymerase (DNA) iota |
| Locus: |
18q21, 2 |
| Discovery year: |
2000-01-24 |
| Entrez gene record: |
11201 |
| Pubmed identfication: |
17609217 |
| RefSeq identity: |
NM_007195 |
| Classification: |
DNA polymerases |
| Havana BLAST/BLAT: |
OTTHUMG00000132704 |