| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DNA polymerase eta |
| Specificity: |
This antibody reacts specifically with DNA polymerase eta |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DNA polymerase eta |
| Gene ID: |
5429 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Depending on the context, Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, May play a role in hypermutation at immunoglobulin genes, Plays an important role in the repair of UV-induced pyrimidine dimers, Plays an important role in translesion synthesis, Targets POLI to replication foci, but causes frequent base transitions and transversions, but does not have lyase activity, it inserts the correct base, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls, DNA polymerase specifically involved in DNA repair |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RAD30A XP-V |
| Also known as: |
DNA polymerase eta Polyclonal Antibody |
| Other name: |
Anti-DNA polymerase eta Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DNA polymerase eta |
| Short name: |
Anti-DNA polymerase eta (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Desoxyribonucleic acid polymerizing enzyme eta (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9181 |
| Gene: |
POLH |
More about : POLH |
| Long gene name: |
DNA polymerase eta |
| Synonyms gene name: |
eta polymerase (DNA) eta , polymerase (DNA directed) |
| Locus: |
6p21, 1 |
| Discovery year: |
1997-09-12 |
| GenBank acession: |
AB024313 |
| Entrez gene record: |
5429 |
| Pubmed identfication: |
10385124 10398605 |
| RefSeq identity: |
NM_006502 |
| Classification: |
Xeroderma pigmentosum complementation groups |
| Havana BLAST/BLAT: |
OTTHUMG00000014743 |
| Locus Specific Databases: |
LRG_470 |