| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RGMA |
| Specificity: |
This antibody reacts specifically with RGMA |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Repulsive Guidance Molecule A |
| Gene ID: |
56963 |
| Swiss Prot: |
Q96B86 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, Furthermore, It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway, Regulates cephalic neural tube closure, SMAD5, and SMAD8, and the formation of mature synapses, inhibits neurite outgrowth and cortical neuron branching, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition, Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RGM RGMa |
| Also known as: |
RGMA Polyclonal Antibody |
| Other name: |
Anti-RGMA Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RGMA |
| Short name: |
Anti-RGMA (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RGMA (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30308 |
| Gene: |
RGMA |
More about : RGMA |
| Long gene name: |
repulsive guidance molecule family member a |
| Synonyms gene name: |
RGM domain family, member A |
| Locus: |
15q26, 1 |
| Discovery year: |
2004-02-04 |
| GenBank acession: |
AL390083 |
| Entrez gene record: |
56963 |
| Pubmed identfication: |
15975920 |
| RefSeq identity: |
NM_020211 |
| Havana BLAST/BLAT: |
OTTHUMG00000171781 |