| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
APAF1CT |
| Specificity: |
This antibody reacts specifically with APAF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Apaf-1 |
| Gene ID: |
317 |
| Swiss Prot: |
O14727 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Isoform 6 is less effective in inducing apoptosis, This activation requires ATP, leading to the activation of caspase-3 and apoptosis, Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CED4 APAF-1 |
| Also known as: |
APAF1 Polyclonal Antibody |
| Other name: |
Anti-APAF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
APAF1 |
| Short name: |
Anti-APAF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-apoptotic peptidase activating factor 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ADP binding, APAF-1 and CED4, APAF1 and IDBG-52364 and ENSG00000120868 and 317, APAF1 and IDBG-636787 and ENSBTAG00000021661 and 537782, Apaf1 and IDBG-185032 and ENSMUSG00000019979 and 11783, nuclei, this GO :0000166 and nucleotide binding and molecular function this GO :0001843 and neural tube closure and biological process this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006952 and defense response and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007399 and nervous system development and biological process this GO :0007420 and brain development and biological process this GO :0008635 and activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c and biological process this GO :0008656 and cysteine-type endopeptidase activator activity involved in apoptotic process and molecular function this GO :0030900 and forebrain development and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043293 and apoptosome and cellular component this GO :0043531 and ADP binding and molecular function this GO :0051402 and neuron apoptotic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072432 and response to G1 DNA damage checkpoint signaling and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1902510 and regulation of apoptotic DNA fragmentation and biological process this GO :2001235 and positive regulation of apoptotic signaling pathway and biological process, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008656 : cysteine-type endopeptidase activator activity involved in apoptotic process and also this GO :0042802 : identical protein binding and also this GO :0043531 : ADP binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008656 : cysteine-type endopeptidase activator activity involved in apoptotic process, this GO :0042802 : identical protein binding, this GO :0043531 : ADP binding, apoptotic peptidase activating factor 1 |
| Identity: |
576 |
| Gene: |
APAF1 |
More about : APAF1 |
| Long gene name: |
apoptotic peptidase activating factor 1 |
| Synonyms gene name: |
apoptotic protease activating factor apoptotic peptidase activating factor |
| Locus: |
12q23, 1 |
| Discovery year: |
1998-01-20 |
| GenBank acession: |
AF013263 |
| Entrez gene record: |
317 |
| Pubmed identfication: |
9267021 10702682 |
| RefSeq identity: |
NM_181861, 1 |
| Classification: |
Caspase recruitment domain containing Apoptosome WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000170214 |