| Also known as: |
CD3 |
| Other names: |
CD3 antigen, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, epsilon polypeptide, T-cell surface glycoprotein CD3 epsilon chain |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD3 |
| Other gene names: |
AI504783, CD3, CD3epsilon, Cd3e, N/A, T3e, Cd3e |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG |
| Clone: |
145-2C11 |
| Host organism: |
Hamster |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
CD3e is a member of the CD3 complex which consists of four subunits, It interacts non-covalently with the CD3 dimers delta/epsilon, NK-T cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, T cell depletions, TCR plays a critical role in T cell development and function, This particular antibody might cross react with speacies outside of the listed ones, _x000D__x000D_CD3e is primarily expressed on T cells, _x000D__x000D_The 145-2C11 antibody is useful for <, a 20kDa transmembrane protein also known as CD3 or T3, and at different levels on thymocytes during T cell differentiation, and is responsible for ligand recognition, and these are associated to the T cell receptor (TCR), as well as apoptosis induction and <, blocking and activation assays, delta, epsilon and zeta, gamma, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell, /i>, /i>, CD3 Detects CD3 epsilon (CD3e), i>, i>, in vitro<, in vitro< |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
IgG concentration 500ug/1000ul |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
CD3: |
| Short name: |
HAMSTER ANTI MOUSE CD3:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
HAMSTER antibody to MOUSE CD3:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |