| Alternative name 1: |
Clone: IF4/OX-8, Mab anti-Rat, CD3:FITC/CD8:RPE |
| Alternative name 2: |
Clone: IF4/OX-8, Mab antibody-Rat, CD3:FITC/CD8:RPE |
| Alternative name 3: |
Clone: IF4/OX-8, Mab antibody-Rattus norvegicustus norvegicus, CD3:FITC/CD8:RPE |
| Alternative name 4: |
anti-CD3: /CD8:R Clone: 4/OX-8 |
| Alternative name 5: |
CD3: /CD8:R Clone: 4/OX-8 |
| Clonality: |
Monoclonal Antibody |
| Description: |
This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity, Cytotoxic T cells cluster differentiators for flow cytometry |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, When excited at 488 nanometers, accurate-monoclonals FITC is highly sensitive to pH extremes, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Rats are used to make rat monoclonal anti mouse antibodies, Rats , There are less rat- than mouse clones however, Monoclonals of this antigen are available in different clones, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Rattus norvegicus, Mus musculus |
| French translation: |
anticorps |
| Gene target: |
Monoclonal IF4/OX-8, CD3: /CD8:RPE |
| Short name: |
Mouse Monoclonal antibody- Clone: IF4/OX-8, CD3:FITC/CD8:RPE |
| Technique: |
Fluorescein, Monoclonals or monoclonal antibodies, mouses, Mouse |
| Host: |
Rat |
| Label: |
FITC |
| Species: |
Mouses, Rats, Rat |
| Alternative name: |
Mouse monoclonal (Antibody to)-Rat, clonality: IF4/OX-8, CD3:fluorecein/CD8:RPE |
| Alternative technique: |
murine |
| Identity: |
1706 |
| Gene: |
CD8A |
More about : CD8A |
| Long gene name: |
CD8a molecule |
| Synonyms gene: |
CD8 |
| Synonyms gene name: |
CD8 antigen, alpha polypeptide (p32) T-cell surface glycoprotein CD8 alpha chain |
| Locus: |
2p11, 2 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
925 |
| Pubmed identfication: |
1541829 |
| RefSeq identity: |
NM_001768 |
| Classification: |
CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000130265 |
| Locus Specific Databases: |
CD8Abase: Mutation registry for CD8 deficiency LRG_44 |