| Also known as: |
CD3 |
| Other names: |
CD3E antigen, N/A, N/A, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, epsilon polypeptide (TiT3 complex), T-cell surface glycoprotein CD3 epsilon chain |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD3 |
| Other gene names: |
CD3E, N/A, N/A, CD3E |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
2A12, CA17 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Dog |
| Specificity and cross-reactivity: |
CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR, CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily, Clone CA17, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, clone CA17, expressed by thymocytes and mature T lymphocytes, 2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin, 2A12 recognises the canine CD3 cell surface antigen, CD3 Mouse anti Dog CD3 monoclonal antibody |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
CD3: |
| Short name: |
MOUSE Anti-DOG CD3:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
MOUSE antibody to-canine CD3:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |