| Also known as: |
CD3 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD3 |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
FN-18 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rhesus Monkey |
| Specificity and cross-reactivity: |
Clone FN18 has been reported to recognise a polymorphic epitope of the RhT3 antigen, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, the homologue of human CD3, which is expressed by mature T lymphocytes, CD3 This item recognises the rhesus monkey RhT3 antigen |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised) |
| Storage and shipping: |
Following reconstitution For optimal long term storage, If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 12 months from reconstitution, Storage in frost-free freezers is not recommended, Temperature variations in the range between +1C to +7C are tolerable, the antibody is photosensitive and should be protected from light, the antibody should be kept at -20 degrees Celsius, the antibody should be stored undiluted, Prior to reconstitution Keep the antibody refrigerated at +4 degrees Celsius |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
CD3: |
| Short name: |
MOUSE Anti-MONKEY CD3:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
MOUSE antibody to-MONKEY CD3:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |