| Long name: |
Biotin Conjugated, RPE65 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RPE65 |
| Specificity: |
This is a highly specific antibody against RPE65 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human RPE65 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2, RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated, Retinal pigment epithelium-specific protein (RPE65) is a 65 kDa protein belonging to the Î, The soluble form of the protein binds vitamin A, This protein is important in 11-cis retinal production as well as in visual pigment regeneration, ², -carotene dioxygenase family, The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
LCA2 rd12 BCO3 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RPE65 Conjugated |
| Short name: |
Conjugated, RPE65 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, RPE65 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10294 |
| Gene: |
RPE65 |
More about : RPE65 |
| Long gene name: |
RPE65, retinoid isomerohydrolase |
| Synonyms gene: |
RP20 |
| Synonyms gene name: |
retinal pigment epithelium-specific protein 65kDa |
| Synonyms name: |
BCO family, member 3 retinol isomerase all-trans-retinyl-palmitate hydrolase |
| Locus: |
1p31, 3 |
| Discovery year: |
1993-10-04 |
| GenBank acession: |
U18991 |
| Entrez gene record: |
6121 |
| Pubmed identfication: |
8340400 16116091 |
| RefSeq identity: |
NM_000329 |
| Havana BLAST/BLAT: |
OTTHUMG00000009208 |
| Locus Specific Databases: |
Mutations of the RPE65 Gene |