| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RPE65 |
| Specificity: |
This antibody reacts specifically with RPE65 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RPE65 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2, RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated, Retinal pigment epithelium-specific protein (RPE65) is a 65 kDa protein belonging to the Î, The soluble form of the protein binds vitamin A, This protein is important in 11-cis retinal production as well as in visual pigment regeneration, ², -carotene dioxygenase family, The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
LCA2 rd12 BCO3 |
| Also known as: |
RPE65 Polyclonal Antibody |
| Other name: |
Anti-RPE65 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RPE65 |
| Short name: |
Anti-RPE65 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RPE65 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10294 |
| Gene: |
RPE65 |
More about : RPE65 |
| Long gene name: |
RPE65, retinoid isomerohydrolase |
| Synonyms gene: |
RP20 |
| Synonyms gene name: |
retinal pigment epithelium-specific protein 65kDa |
| Synonyms name: |
BCO family, member 3 retinol isomerase all-trans-retinyl-palmitate hydrolase |
| Locus: |
1p31, 3 |
| Discovery year: |
1993-10-04 |
| GenBank acession: |
U18991 |
| Entrez gene record: |
6121 |
| Pubmed identfication: |
8340400 16116091 |
| RefSeq identity: |
NM_000329 |
| Havana BLAST/BLAT: |
OTTHUMG00000009208 |
| Locus Specific Databases: |
Mutations of the RPE65 Gene |