| Long name: |
FITC Conjugated, CstF-64 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CstF-64 |
| Specificity: |
This is a highly specific antibody against CstF-64 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human CSTF2T/CstF-64 |
| Gene ID number: |
23283 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF, CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, However, The first step, The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, a heterotrimer that is composed of three distinct subunits, a large variant of CstF-64, and promotes the germ cell pattern of polyadenylation, but not meiotic, called t CstF-64, endonucleolytic cleavage of polyadenylation substrates, germ cells, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, requires CstF (cleavage stimulation factor), suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation, whereas t CstF-64 is encoded by an autosomal gene, Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CSTF 64 kDa subunit, CSTFT_HUMAN, Cleavage stimulation factor 64 kDa subunit, KIAA0689, TauCstF64, tau variant, tau variant, tau variant, CF1 64 kDa subunit |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CstF-64 Conjugated |
| Short name: |
FITC Conjugated, CstF-64 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, CstF-64 (Antibody to) |
| Alternative technique: |
antibodies |