| Long name: |
Biotin Conjugated, PEA3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PEA3 |
| Specificity: |
This is a highly specific antibody against PEA3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human PEA3 |
| Gene ID number: |
2118 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Drosophila E74, Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element, Ets-2 and Fli-1, For instance, It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells, These include mouse PU, This is in contrast to other members of the Ets gene family, although PU, but does not bind to the sequence 5'-AGGAAC-3', chicken and human Ets-2 and rat GABP-a, each of which is expressed primarily in cells of hematopoietic origin, mouse and human Ets-1, recognized by PU, such as Ets-1, 1, 1, 1 binds equally well to both sequences, Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
E1A-F E1AF PEA3 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PEA3 Conjugated |
| Short name: |
Conjugated, PEA3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, PEA3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3493 |
| Gene: |
ETV4 |
More about : ETV4 |
| Long gene name: |
ETS variant 4 |
| Synonyms gene name: |
E1AF) , ets variant gene 4 (E1A enhancer-binding protein |
| Synonyms name: |
E1A enhancer binding protein |
| Locus: |
17q21, 31 |
| Discovery year: |
1995-03-27 |
| GenBank acession: |
U18018 |
| Entrez gene record: |
2118 |
| Pubmed identfication: |
8530053 1547944 |
| RefSeq identity: |
NM_001986 |
| Classification: |
ETS transcription factor family |
| Havana BLAST/BLAT: |
OTTHUMG00000180884 |