| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PEA3 |
| Specificity: |
This antibody reacts specifically with PEA3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PEA3 |
| Gene ID: |
2118 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Drosophila E74, Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element, Ets-2 and Fli-1, For instance, It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells, These include mouse PU, This is in contrast to other members of the Ets gene family, although PU, but does not bind to the sequence 5'-AGGAAC-3', chicken and human Ets-2 and rat GABP-a, each of which is expressed primarily in cells of hematopoietic origin, mouse and human Ets-1, recognized by PU, such as Ets-1, 1, 1, 1 binds equally well to both sequences, Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
E1A-F E1AF PEA3 |
| Also known as: |
PEA3 Polyclonal Antibody |
| Other name: |
Anti-PEA3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PEA3 |
| Short name: |
Anti-PEA3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PEA3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3493 |
| Gene: |
ETV4 |
More about : ETV4 |
| Long gene name: |
ETS variant 4 |
| Synonyms gene name: |
E1AF) , ets variant gene 4 (E1A enhancer-binding protein |
| Synonyms name: |
E1A enhancer binding protein |
| Locus: |
17q21, 31 |
| Discovery year: |
1995-03-27 |
| GenBank acession: |
U18018 |
| Entrez gene record: |
2118 |
| Pubmed identfication: |
8530053 1547944 |
| RefSeq identity: |
NM_001986 |
| Classification: |
ETS transcription factor family |
| Havana BLAST/BLAT: |
OTTHUMG00000180884 |