| Long name: |
FITC Conjugated, Sema4C Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Sema4C |
| Specificity: |
This is a highly specific antibody against Sema4C |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human Sema4C |
| Gene ID number: |
9037 |
| Swiss Prot: |
Q13591 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
In glioma cells, Ligand for receptor PLXNB3, May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1, while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity), Bifunctional axonal guidance cue regulated by sulfated proteoglycans |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SEMA5A, SEMAF, Sema F, Semaphorin-5A, Semaphorin-F, semF |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Sema4C Conjugated |
| Short name: |
FITC Conjugated, Sema4C Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
(semaphorin) 4C (Antibody to), fluorecein coupled, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Alternative technique: |
antibodies |