| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Sema4C |
| Specificity: |
This antibody reacts specifically with Sema4C |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Sema4C |
| Gene ID: |
9037 |
| Swiss Prot: |
Q13591 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In glioma cells, Ligand for receptor PLXNB3, May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1, while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity), Bifunctional axonal guidance cue regulated by sulfated proteoglycans |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Semacl1 Semaf |
| Also known as: |
Sema4C Polyclonal Antibody |
| Other name: |
Anti-Sema4C Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Sema4C |
| Short name: |
Anti-Sema4C (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
(semaphorin) 4C (polyclonal), ALEXA Fluor 594, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, antibody to-sema domain |
| Alternative technique: |
polyclonals |
| Identity: |
10731 |
| Gene: |
SEMA4C |
More about : SEMA4C |
| Long gene name: |
semaphorin 4C |
| Synonyms gene: |
SEMAI |
| Synonyms gene name: |
(semaphorin) 4C , immunoglobulin domain (Ig), sema domain, transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms name: |
M-Sema F |
| Locus: |
2q11, 2 |
| Discovery year: |
2004-02-04 |
| GenBank acession: |
AB051526 |
| Entrez gene record: |
54910 |
| Pubmed identfication: |
7656991 |
| RefSeq identity: |
NM_017789 |
| Classification: |
Semaphorins Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000130535 |