| Long name: |
FITC Conjugated, GAB4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GAB4 |
| Specificity: |
This is a highly specific antibody against GAB4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human GAB4 |
| Gene ID number: |
128954 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cytokine/growth factor triggering of protein tyrosine kinase receptors (RTKs) initiates signaling cascades that progress to the nucleus where signals for activation, Gab 1-4 contain Pleckstrin homology and potential binding sites for SH2 and SH3 domain-containing proteins, Gab 4 (GRB2-associated-binding protein 4), Insulin receptor and EGF receptor signaling are among the cascades that rely on Gab family members to elicit a nuclear response to an extracellular stimulus, The recruitment of signaling partners to Gab family members is phosphorylation-dependent, This scaffolding mechanism represents a critical link in cytokine/growth factor signaling routes, also designated GRB2-associated-binding protein 2-like (Gab 2-like), is a 574 amino acid protein that shares 62% sequence similarity with Gab 2 and contains one Pleckstrin homology domain, proliferation and differentiation occur, The Gab family of adaptor proteins function as molecular scaffolds that mediate protein recruit-ment to RTKs |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GAB4 Conjugated |
| Short name: |
FITC Conjugated, GAB4 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, member 4 (Antibody to), GRB2-associated binding protein family |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
GAB2 and IDBG-642116 and ENSBTAG00000002922 and 529346, GAB4 and IDBG-227875 and ENSG00000215568 and 128954, Gab2 and IDBG-199566 and ENSMUSG00000004508 and 14389, member 4, multiple, GRB2-associated binding protein family |
| Identity: |
18325 |
| Gene: |
GAB4 |
More about : GAB4 |
| Long gene name: |
GRB2 associated binding protein family member 4 |
| Synonyms gene name: |
GRB2-associated binding protein family, member 4 |
| Locus: |
22q11, 1 |
| Discovery year: |
2004-07-22 |
| GenBank acession: |
AK057252 |
| Entrez gene record: |
128954 |
| RefSeq identity: |
XM_372882 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000149992 |