| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GAB4 |
| Specificity: |
This antibody reacts specifically with GAB4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GAB4 |
| Gene ID: |
128954 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Cytokine/growth factor triggering of protein tyrosine kinase receptors (RTKs) initiates signaling cascades that progress to the nucleus where signals for activation, Gab 1-4 contain Pleckstrin homology and potential binding sites for SH2 and SH3 domain-containing proteins, Gab 4 (GRB2-associated-binding protein 4), Insulin receptor and EGF receptor signaling are among the cascades that rely on Gab family members to elicit a nuclear response to an extracellular stimulus, The recruitment of signaling partners to Gab family members is phosphorylation-dependent, This scaffolding mechanism represents a critical link in cytokine/growth factor signaling routes, also designated GRB2-associated-binding protein 2-like (Gab 2-like), is a 574 amino acid protein that shares 62% sequence similarity with Gab 2 and contains one Pleckstrin homology domain, proliferation and differentiation occur, The Gab family of adaptor proteins function as molecular scaffolds that mediate protein recruit-ment to RTKs |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
GAB4 Polyclonal Antibody |
| Other name: |
Anti-GAB4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GAB4 |
| Short name: |
Anti-GAB4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, member 4 (polyclonal), antibody to-GRB2-associated binding protein family |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
GAB2 and IDBG-642116 and ENSBTAG00000002922 and 529346, GAB4 and IDBG-227875 and ENSG00000215568 and 128954, Gab2 and IDBG-199566 and ENSMUSG00000004508 and 14389, member 4, multiple, GRB2-associated binding protein family |
| Identity: |
18325 |
| Gene: |
GAB4 |
More about : GAB4 |
| Long gene name: |
GRB2 associated binding protein family member 4 |
| Synonyms gene name: |
GRB2-associated binding protein family, member 4 |
| Locus: |
22q11, 1 |
| Discovery year: |
2004-07-22 |
| GenBank acession: |
AK057252 |
| Entrez gene record: |
128954 |
| RefSeq identity: |
XM_372882 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000149992 |