| Long name: |
FITC Conjugated, FZD3/Frizzled 3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
FZD3/Frizzled 3 |
| Specificity: |
This is a highly specific antibody against FZD3/Frizzled 3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
150-200/666 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3 |
| Gene ID number: |
7976 |
| Swiss Prot: |
Q9NPG1 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, Both pathways seem to involve interactions with G-proteins, May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues, Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes, which leads to the activation of disheveled proteins, Receptor for Wnt proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
FZD3/Frizzled 3 Conjugated |
| Short name: |
FITC Conjugated, FZD3/Frizzled 3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, FZD3/Frizzled 3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4041 |
| Gene: |
FZD3 |
More about : FZD3 |
| Long gene name: |
frizzled class receptor 3 |
| Synonyms gene name: |
frizzled (Drosophila) homolog 3 frizzled homolog 3 (Drosophila) frizzled 3, seven transmembrane spanning receptor frizzled family receptor 3 |
| Locus: |
8p21, 1 |
| Discovery year: |
1998-09-17 |
| GenBank acession: |
AJ272427 |
| Entrez gene record: |
7976 |
| Pubmed identfication: |
10777673 10873558 |
| RefSeq identity: |
NM_145866 |
| Classification: |
Class F frizzled , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000102145 |