| Long name: |
Cy3 Conjugated, Brk (Tyr447) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Brk Tyr447 |
| Specificity: |
This is a highly specific antibody against Brk Tyr447 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr447 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
438-451/451 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Brk around the phosphorylation site of Tyr447 [TS(p-Y)EN] |
| Gene ID number: |
5753 |
| Swiss Prot: |
Q13882 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A number of potential nuclear and cytoplasmic substrates have been identified, Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, Association with EGFR, BTK/ATK, Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p19RhoGAP, EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, EGFR, ERBB2, ERBB2, ERBB3 and IRS4, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase, Function seems to be context dependent and differ depending on cell type, In normal or non-tumorigenic tissues, In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF, Nuclear PTK6 may be important for regulating growth in normal epithelia, PTK6 activates STAT3 and STAT5B to promote proliferation, PTK6 promotes cellular differentiation and apoptosis, PXN/paxillin, STAP2/BKS, These include the RNA-binding proteins: KHDRBS1/SAM68, These proteins include ADAM15, anchorage-independent survival and migration/invasion, as well as its intracellular localization, as well as tumor growth, inactivating RhoA while activating RAS, or for which PTK6 may play an adapter-like role, thereby promoting migration and invasion, transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p19RhoGAP, which promotes association with RASA1/p12RasGAP, while cytoplasmic PTK6 might activate oncogenic signaling pathways, Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
Breast tumor kinase, PTK6, Protein-tyrosine kinase 6, Tyrosine-protein kinase BRK, BRK |
| Properties: |
1 of the Brk (Tyr447) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Brk (Tyr447) Conjugated |
| Short name: |
Conjugated, Brk (Tyr447) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, Brk (Tyr447) (Antibody to) |
| Alternative technique: |
antibodies |