| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Brk Tyr447 |
| Specificity: |
This antibody reacts specifically with Brk (Tyr447) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr447 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Brk around the phosphorylation site of Tyr447 [TS(p-Y)EN] |
| Gene ID: |
5753 |
| Swiss Prot: |
Q13882 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A number of potential nuclear and cytoplasmic substrates have been identified, Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, Association with EGFR, BTK/ATK, Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p19RhoGAP, EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, EGFR, ERBB2, ERBB2, ERBB3 and IRS4, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase, Function seems to be context dependent and differ depending on cell type, In normal or non-tumorigenic tissues, In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF, Nuclear PTK6 may be important for regulating growth in normal epithelia, PTK6 activates STAT3 and STAT5B to promote proliferation, PTK6 promotes cellular differentiation and apoptosis, PXN/paxillin, STAP2/BKS, These include the RNA-binding proteins: KHDRBS1/SAM68, These proteins include ADAM15, anchorage-independent survival and migration/invasion, as well as its intracellular localization, as well as tumor growth, inactivating RhoA while activating RAS, or for which PTK6 may play an adapter-like role, thereby promoting migration and invasion, transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p19RhoGAP, which promotes association with RASA1/p12RasGAP, while cytoplasmic PTK6 might activate oncogenic signaling pathways, Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Breast tumor kinase, PTK6, Protein-tyrosine kinase 6, Tyrosine-protein kinase BRK, BRK |
| Also known as: |
Brk (Tyr447) Polyclonal Antibody |
| Other name: |
Anti-Brk (Tyr447) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Brk (Tyr447) |
| Short name: |
Anti-Brk (Tyr447) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Brk (Tyr447) (polyclonal) |
| Alternative technique: |
polyclonals |