| Long name: |
Cy5 Conjugated, Brk (Tyr447) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Brk Tyr447 |
| Specificity: |
This is a highly specific antibody against Brk Tyr447 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr447 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
438-451/451 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Brk around the phosphorylation site of Tyr447 [TS(p-Y)EN] |
| Gene ID number: |
5753 |
| Swiss Prot: |
Q13882 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A number of potential nuclear and cytoplasmic substrates have been identified, Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, Association with EGFR, BTK/ATK, Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p19RhoGAP, EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, EGFR, ERBB2, ERBB2, ERBB3 and IRS4, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase, Function seems to be context dependent and differ depending on cell type, In normal or non-tumorigenic tissues, In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF, Nuclear PTK6 may be important for regulating growth in normal epithelia, PTK6 activates STAT3 and STAT5B to promote proliferation, PTK6 promotes cellular differentiation and apoptosis, PXN/paxillin, STAP2/BKS, These include the RNA-binding proteins: KHDRBS1/SAM68, These proteins include ADAM15, anchorage-independent survival and migration/invasion, as well as its intracellular localization, as well as tumor growth, inactivating RhoA while activating RAS, or for which PTK6 may play an adapter-like role, thereby promoting migration and invasion, transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p19RhoGAP, which promotes association with RASA1/p12RasGAP, while cytoplasmic PTK6 might activate oncogenic signaling pathways, Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
625, 650nm/670nm |
| Synonyms: |
Breast tumor kinase, PTK6, Protein-tyrosine kinase 6, Tyrosine-protein kinase BRK, BRK |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Brk (Tyr447) Conjugated |
| Short name: |
Conjugated, Brk (Tyr447) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy5 |
| Alternative name: |
cyanine 5 coupled, Brk (Tyr447) (Antibody to) |
| Alternative technique: |
antibodies |