| Long name: |
FITC Conjugated, BIG1/ARFGEF1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BIG1/ARFGEF1 |
| Specificity: |
This is a highly specific antibody against BIG1/ARFGEF1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1 |
| Gene ID number: |
10565 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol, BIG2 associates with PKA regulatory subunits, Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity, GEPs comprise two major families, In vivo, Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2, a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking, a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways, large GEPs that are inhibited by brefeldin A (BFA), Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
DKFZP434L057 BIG1 ARFGEP1 p200 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
BIG1/ARFGEF1 Conjugated |
| Short name: |
FITC Conjugated, BIG1/ARFGEF1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, BIG1/ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022, ARFGEP1 and BIG1 and P200, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, nuclei, protein kinase A regulatory subunit binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005086 and ARF guanyl-nucleotide exchange factor activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0005829 and cytosol and cellular component this GO :0006887 and exocytosis and biological process this GO :0007030 and this GO lgi organization and biological process this GO :0010256 and endomembrane system organization and biological process this GO :0015031 and protein transport and biological process this GO :0016192 and vesicle-mediated transport and biological process this GO :0016363 and nuclear matrix and cellular component this GO :0017022 and myosin binding and molecular function this GO :0030532 and small nuclear ribonucleoprotein complex and cellular component this GO :0030837 and negative regulation of actin filament polymerization and biological process this GO :0032012 and regulation of ARF protein signal transduction and biological process this GO :0034237 and protein kinase A regulatory subunit binding and molecular function this GO :0034259 and negative regulation of Rho GTPase activity and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0090284 and positive regulation of protein glycosylation in this GO lgi and biological process this GO :0090303 and positive regulation of wound healing and biological process this GO :2000114 and regulation of establishment of cell polarity and biological process, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005086 : ARF guanyl-nucleotide exchange factor activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0017022 : myosin binding and also this GO :0034237 : protein kinase A regulatory subunit binding, this GO :0005086 : ARF guanyl-nucleotide exchange factor activity, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0017022 : myosin binding, this GO :0034237 : protein kinase A regulatory subunit binding, ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) |
| Identity: |
15772 |
| Gene: |
ARFGEF1 |
More about : ARFGEF1 |
| Long gene name: |
ADP ribosylation factor guanine nucleotide exchange factor 1 |
| Synonyms gene name: |
ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) |
| Synonyms name: |
Brefeldin A-inhibited guanine nucleotide-exchange protein 1 |
| Locus: |
8q13, 2 |
| Discovery year: |
2004-08-09 |
| GenBank acession: |
AF084520 |
| Entrez gene record: |
10565 |
| Pubmed identfication: |
10212200 8917509 |
| RefSeq identity: |
NM_006421 |
| Havana BLAST/BLAT: |
OTTHUMG00000164626 |