| Long name: |
ALEXA FLUOR 488 Conjugated, BIG1/ARFGEF1 Polyclonal Antibody |
| Also known as: |
Anti-BIG1/ARFGEF1 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BIG1/ARFGEF1 |
| Specificity: |
This is a highly specific antibody against BIG1/ARFGEF1 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1 |
| Gene ID Number: |
10565 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol, BIG2 associates with PKA regulatory subunits, Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity, GEPs comprise two major families, In vivo, Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2, a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking, a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways, large GEPs that are inhibited by brefeldin A (BFA), Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs) |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
DKFZP434L057 BIG1 ARFGEP1 p200 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the BIG1/ARFGEF1 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
BIG1/ARFGEF1 |
| Short name: |
BIG1/ARFGEF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, BIG1/ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022, ARFGEP1 and BIG1 and P200, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, nuclei, protein kinase A regulatory subunit binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005086 and ARF guanyl-nucleotide exchange factor activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0005829 and cytosol and cellular component this GO :0006887 and exocytosis and biological process this GO :0007030 and this GO lgi organization and biological process this GO :0010256 and endomembrane system organization and biological process this GO :0015031 and protein transport and biological process this GO :0016192 and vesicle-mediated transport and biological process this GO :0016363 and nuclear matrix and cellular component this GO :0017022 and myosin binding and molecular function this GO :0030532 and small nuclear ribonucleoprotein complex and cellular component this GO :0030837 and negative regulation of actin filament polymerization and biological process this GO :0032012 and regulation of ARF protein signal transduction and biological process this GO :0034237 and protein kinase A regulatory subunit binding and molecular function this GO :0034259 and negative regulation of Rho GTPase activity and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0090284 and positive regulation of protein glycosylation in this GO lgi and biological process this GO :0090303 and positive regulation of wound healing and biological process this GO :2000114 and regulation of establishment of cell polarity and biological process, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005086 : ARF guanyl-nucleotide exchange factor activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0017022 : myosin binding and also this GO :0034237 : protein kinase A regulatory subunit binding, this GO :0005086 : ARF guanyl-nucleotide exchange factor activity, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0017022 : myosin binding, this GO :0034237 : protein kinase A regulatory subunit binding, ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) |
| Identity: |
15772 |
| Gene: |
ARFGEF1 |
More about : ARFGEF1 |
| Long gene name: |
ADP ribosylation factor guanine nucleotide exchange factor 1 |
| Synonyms gene name: |
ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) |
| Synonyms name: |
Brefeldin A-inhibited guanine nucleotide-exchange protein 1 |
| Locus: |
8q13, 2 |
| Discovery year: |
2004-08-09 |
| GenBank acession: |
AF084520 |
| Entrez gene record: |
10565 |
| Pubmed identfication: |
10212200 8917509 |
| RefSeq identity: |
NM_006421 |
| Havana BLAST/BLAT: |
OTTHUMG00000164626 |