| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BIG1/ARFGEF1 |
| Specificity: |
This antibody reacts specifically with BIG1/ARFGEF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1 |
| Gene ID: |
10565 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol, BIG2 associates with PKA regulatory subunits, Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity, GEPs comprise two major families, In vivo, Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2, a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking, a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways, large GEPs that are inhibited by brefeldin A (BFA), Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DKFZP434L057 BIG1 ARFGEP1 p200 |
| Also known as: |
BIG1/ARFGEF1 Polyclonal Antibody |
| Other name: |
Anti-BIG1/ARFGEF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BIG1/ARFGEF1 |
| Short name: |
Anti-BIG1/ARFGEF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-BIG1/ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ARFGEF1 and IDBG-24344 and ENSG00000066777 and 10565, ARFGEF1 and IDBG-644666 and ENSBTAG00000014691 and 281022, ARFGEP1 and BIG1 and P200, Arfgef1 and IDBG-133303 and ENSMUSG00000067851 and 211673, nuclei, protein kinase A regulatory subunit binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005086 and ARF guanyl-nucleotide exchange factor activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0005829 and cytosol and cellular component this GO :0006887 and exocytosis and biological process this GO :0007030 and this GO lgi organization and biological process this GO :0010256 and endomembrane system organization and biological process this GO :0015031 and protein transport and biological process this GO :0016192 and vesicle-mediated transport and biological process this GO :0016363 and nuclear matrix and cellular component this GO :0017022 and myosin binding and molecular function this GO :0030532 and small nuclear ribonucleoprotein complex and cellular component this GO :0030837 and negative regulation of actin filament polymerization and biological process this GO :0032012 and regulation of ARF protein signal transduction and biological process this GO :0034237 and protein kinase A regulatory subunit binding and molecular function this GO :0034259 and negative regulation of Rho GTPase activity and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0090284 and positive regulation of protein glycosylation in this GO lgi and biological process this GO :0090303 and positive regulation of wound healing and biological process this GO :2000114 and regulation of establishment of cell polarity and biological process, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005086 : ARF guanyl-nucleotide exchange factor activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0017022 : myosin binding and also this GO :0034237 : protein kinase A regulatory subunit binding, this GO :0005086 : ARF guanyl-nucleotide exchange factor activity, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0017022 : myosin binding, this GO :0034237 : protein kinase A regulatory subunit binding, ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) |
| Identity: |
15772 |
| Gene: |
ARFGEF1 |
More about : ARFGEF1 |
| Long gene name: |
ADP ribosylation factor guanine nucleotide exchange factor 1 |
| Synonyms gene name: |
ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) |
| Synonyms name: |
Brefeldin A-inhibited guanine nucleotide-exchange protein 1 |
| Locus: |
8q13, 2 |
| Discovery year: |
2004-08-09 |
| GenBank acession: |
AF084520 |
| Entrez gene record: |
10565 |
| Pubmed identfication: |
10212200 8917509 |
| RefSeq identity: |
NM_006421 |
| Havana BLAST/BLAT: |
OTTHUMG00000164626 |