| Long name: |
FITC Conjugated, THEMIS Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
THEMIS |
| Specificity: |
This is a highly specific antibody against THEMIS |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
300-350/641 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human THEMIS |
| Gene ID number: |
387357 |
| Swiss Prot: |
Q8N1K5 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk (By similarity), Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells, Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
bA325O24, 3 , 4 FLJ40584 bA325O24 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
THEMIS Conjugated |
| Short name: |
FITC Conjugated, THEMIS Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, thymocyte selection associated (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
THEMIS and IDBG-632941 and ENSBTAG00000015615 and 540554, THEMIS and IDBG-96262 and ENSG00000172673 and 387357, Themis and IDBG-141613 and ENSMUSG00000049109 and 210757, nuclei, protein binding, this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0008180 and COP9 signalosome and cellular component this GO :0043368 and positive T cell selection and biological process this GO :0043383 and negative T cell selection and biological process this GO :0050852 and T cell receptor signaling pathway and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, thymocyte selection associated |
| Identity: |
21569 |
| Gene: |
THEMIS |
More about : THEMIS |
| Long gene name: |
thymocyte selection associated |
| Synonyms gene: |
C6orf207 C6orf190 TSEPA |
| Synonyms gene name: |
chromosome 6 open reading frame 207 chromosome 6 open reading frame 190 thymocyte selection pathway associated |
| Synonyms name: |
thymocyte expressed molecule involved in selection |
| Locus: |
6q22, 33 |
| Discovery year: |
2003-11-25 |
| GenBank acession: |
AK094863 |
| Entrez gene record: |
387357 |
| Pubmed identfication: |
19597499 19597498 19597497 |
| RefSeq identity: |
NM_001010923 |
| Havana BLAST/BLAT: |
OTTHUMG00000015534 |