| Long name: |
FITC Conjugated, SLC5A3/SMIT Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SLC5A3/SMIT |
| Specificity: |
This is a highly specific antibody against SLC5A3/SMIT |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human SLC5A3/SMIT |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Impaired SMIT activity is implicated in the pathogenesis of diabetes and Down syndrome, In kidney, It is expressed in many human tissues, It is taken up into cells by the sodium/myo-inositol cotransporter (SMIT), SMIT activity maintains intracellular concentrations of myo-inositol, SMIT is abundantly expressed throughout the whole brain and spinal cord in fetal rat, SMIT localizes to the baso-lateral membranes of the thick ascending limb of Henle (TAL) and the inner medullary collecting duct (IMCD), Specifically, The human SMIT protein is encoded by the SLC5A3 gene, but is downregulated in adult rat brain with the exception of the choroid plexus, it is upregulated in response to hypertonic stress, kidney and placenta, signal transduction and osmoregulation, such as brain, where SMIT expression remains high, which maps to chromosome 21q22, 12, Myo-inositol is involved in many important aspects of cellular regulation including membrane structure |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SMIT SMIT1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
SLC5A3/SMIT Conjugated |
| Short name: |
FITC Conjugated, SLC5A3/SMIT Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, SLC5A3/SMIT (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11038 |
| Gene: |
SLC5A3 |
More about : SLC5A3 |
| Long gene name: |
solute carrier family 5 member 3 |
| Synonyms gene name: |
member 3 , member 3 solute carrier family 5 (sodium/myo-inositol cotransporter), solute carrier family 5 (inositol transporter) |
| Locus: |
21q22, 11 |
| Discovery year: |
1994-02-15 |
| Entrez gene record: |
6526 |
| Pubmed identfication: |
7789985 |
| Classification: |
Solute carriers |
| Havana BLAST/BLAT: |
OTTHUMG00000065821 |