| Long name: |
FITC Conjugated, OTP Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
OTP |
| Specificity: |
This is a highly specific antibody against OTP |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human OTP |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
At early embryonic stages in mice, Containing a homeobox DNA-binding domain and a OAR domain, OTP (orthopedia homeobox) is a 325 amino acid nuclear protein belonging to the paired homeobox family and Bicoid subfamily, OTP is expressed in neurons, OTP is highly conserved in evolution and is encoded by a gene located on human chromosome 5, OTP is suggested to be involved in the differentiation of hypothalamic neuroendocrine cells, anterior periventricular (aPV) and arcuate (ARN) nuclei, indicating a potential role for OTP as an upstream regulator of SIM2, supraoptic (SON), the expression of SIM2 is downregulated in the absence of OTP, which contains 181 million base pairs and comprises nearly 6% of the human genome, which give rise to the paraventricular (PVN), Homeodomain (HD) genes are helix-turn-helix transcription factors that play key roles in the specification of cell fates |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
OTP Conjugated |
| Short name: |
FITC Conjugated, OTP Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, OTP (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8518 |
| Gene: |
OTP |
More about : OTP |
| Long gene name: |
orthopedia homeobox |
| Synonyms gene name: |
orthopedia homolog (Drosophila) |
| Locus: |
5q14, 1 |
| Discovery year: |
1999-02-09 |
| Entrez gene record: |
23440 |
| Pubmed identfication: |
10458915 |
| Classification: |
PRD class homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000102171 |