| Long name: |
FITC Conjugated, Gli3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Gli3 |
| Specificity: |
This is a highly specific antibody against Gli3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Gli3 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, Mutations in the ptc gene, The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning, The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins, The ptc gene, also identified as a Drosophila segment polarity gene, basal cell carcinoma, encodes the transmembrane protein patched, have been correlated with the most common type of cancer, however, the expression of which is precisely regulated during embryonic development, the molecular basis for this transformation was poorly understood, which affects 750, which result in unregulated hedgehog signaling, while patched functions as a repressor opposing the effects of hedgehog, 000 individuals annually in the United States alone, It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
DNA binding protein, GCPS, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3, GLI Kruppel family member GLI3 Greig cephalopolysyndactyly syndrome, GLI family zinc finger 3, GLI3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3FL, GLI3_HUMAN, Gli 3, Glioma associated oncogene family zinc finger 3, Oncogene GLI3, PAP A, PAPA, PAPA 1, PAPA1, PAPB, PHS, PPD IV, PPDIV, Transcriptional activator GLI3, Transcriptional repressor GLI3R, Zinc finger protein GLI 3, Zinc finger protein GLI3, ACLS |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Gli3 Conjugated |
| Short name: |
FITC Conjugated, Gli3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, GLI family zinc finger 3 (Antibody to) |
| Alternative technique: |
antibodies |