| Long name: |
ALEXA FLUOR 594 Conjugated, Gli3 Polyclonal Antibody |
| Also known as: |
Anti-Gli3 PAb ALEXA FLUOR 594 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Gli3 |
| Specificity: |
This is a highly specific antibody against Gli3 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Gli3 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, Mutations in the ptc gene, The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning, The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins, The ptc gene, also identified as a Drosophila segment polarity gene, basal cell carcinoma, encodes the transmembrane protein patched, have been correlated with the most common type of cancer, however, the expression of which is precisely regulated during embryonic development, the molecular basis for this transformation was poorly understood, which affects 750, which result in unregulated hedgehog signaling, while patched functions as a repressor opposing the effects of hedgehog, 000 individuals annually in the United States alone, It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PAP-A PAPA PAPA1 PAPB ACLS PPDIV GLI3 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
594, ALEXA FLUOR®, Alexa Fluor |
| Conjugated: |
Alexa conjugate 1 |
| Gene target: |
Gli3 |
| Short name: |
Gli3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 594 |
| Alternative name: |
ALEXA FLUOR 594, GLI family zinc finger 3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4207 |
| Gene: |
GCPS |
More about : GCPS |
| Long gene name: |
GLI family zinc finger 3 |
| Synonyms gene: |
PHS |
| Synonyms gene name: |
Greig cephalopolysyndactyly syndrome GLI-Kruppel family member GLI3 glioma-associated oncogene family zinc finger 3 |
| Synonyms name: |
zinc finger protein GLI3 oncogene GLI3 DNA-binding protein |
| Locus: |
7p14, 1 |
| Discovery year: |
1989-05-29 |
| Entrez gene record: |
2737 |
| Pubmed identfication: |
2118997 |
| RefSeq identity: |
NM_000168 |
| Classification: |
Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000023630 |